Amino acid selective pulse sequences for an automated assignment of protein spectra


Triple-resonance experiments are routinely used for the assignment of protein spectra. This is mainly due to their high sensitivity, their intrinsically good resolution and their independence from the three-dimensional structure of the molecule under investigation. Moreover, the ease of interpretation makes these spectra ideally suited for an automated or semi-automated assignment procedures. Goal of this project is the development of a modified strategy for sequence specific assignment based on new amino acid selective pulse sequences and its implementation in a routine for automated assignment.

Publications
M. Schubert; D. Labudde; D. Leitner; H. Oschkinat; P. Schmieder*; "A modified strategy for sequence specific assignment of protein NMR spectra based on amino acid type selective experiments"; J. Biomol NMR 31, 115-128 (2005)

DOI: 10.1007/s10858-004-8263-z
M. Schubert; H. Oschkinat; P. Schmieder*; "MUSIC and aromatic residues: Amino acid type-selective 1H-15N correlations, part III"; J. Magn. Reson. 153, 186-192 (2001)

DOI: 10.1006/jmre.2001.2447
M. Schubert; H. Oschkinat; P. Schmieder*; "Amino acid type-selective 1H-15N correlations for Arg and Lys"; J. Biomolec. NMR 20, 379-384 (2001)

DOI: 10.1023/A:1011206131623
M. Schubert; H. Oschkinat; P. Schmieder*; "MUSIC, selective pulses and tuned delays: Amino acid type-selective 1H-15N correlations, part II"; J. Magn. Reson. 148, 61-72 (2001)

DOI: 10.1006/jmre.2000.2222
M. Schubert; L. Ball; H. Oschkinat; P. Schmieder*; "Bridging the gap: A set of selective 1H-15N correlations to link sequential neighbors of proline“; J. Biomolec. NMR 17, 331-335 (2000)

DOI: 10.1023/A:1008362904205
M. Schubert; M. Smalla; P. Schmieder*; H. Oschkinat; "MUSIC in triple resonance experiments: Amino acid type selective 1H-15N correlations"; J. Magn. Reson. 141, 34-43 (1999)

DOI: 10.1006/jmre.1999.1881
P. Schmieder*; M. Leidert; M.J.S. Kelly; H. Oschkinat; "Multiplicity-selective coherence transfer steps for the design of amino-acid selective experiments - a triple resonance experiment selective for Asn and Gln"; J. Magn. Reson. 131, 199-201 (1998)

DOI: 10.1006/jmre.1997.1348

last changes 09.03.2013, Peter Schmieder